KMID : 0364820090450020148
|
|
Korean Journal of Microbiology 2009 Volume.45 No. 2 p.148 ~ p.154
|
|
Bacterial Community Dynamics during Composting of Food Wastes
|
|
Shin Ji-Hye
Lee Jin-Woo Nam Ji-Hyun Park Se-Yong Lee Dong-Hun
|
|
Abstract
|
|
|
Composting is a biological process converting solid organic waste into valuable materials such as fertilizer. The change of bacterial populations in a composting reactor of food waste was investigated for 2 months. Based on shifts in temperature profile, the composting process could be divided into the first phase (2oC~55oC), the second phase (55oC~97oC), and the third phase (50oC~89oC). The number of total bacteria was 1.66¡¿1011 cell/g, 0.29¡¿1011 cell/g, and 0.28¡¿1011 cell/g in the first, second, and third stages, respectively. The proportions of thermophiles increased from 33% to 89% in the second stage. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of bacterial community structure was coupled with shifts in composting stages. The structure of bacterial community in the ultra-thermophilic second stage reflected that of seeding starter. The major decomposers driving the ultra-thermophilic composting were identified as phylotypes related to Bacillus and Pseudomonas.
|
|
KEYWORD
|
|
16S rRNA, composting, thermophilic bacteria, T-RFLP
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|